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KMID : 0545119910010020136
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Journal of Microbiology and Biotechnology
1991 Volume.1 No. 2 p.136 ~ p.141
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Optimum Culture Conditions of Brevibacterium sp. CH2 for Production of Nitrile Hydratase
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Choi, Sang Kyo
Lee, Cheo Young/Chang, Ho Nam/Hwang, Jun Sik
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Abstract
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Optimum culture conditions for the formation of nitrite hydratase by Brevibacterium sp. CH2 were investigated. Addition of ferric and ferrous ions greatly increased the nitrile hydratase formation. The effects of nitriles, amides, and acids as an inducer on the formation of nitrite hydratase were investigated. Isobutyramide was the best inducer among the tested compounds. When Brevibacterium sp. CH2 was cultivated for 23 h at 30℃ in a optimized medium containing 15g of glucose, 5g of bacto peptone, 3g of yeast extract, 3g of malt extract, 1g of KH_2PO_4, 19 of K_2HPO_4, 1 g of NaCl, 0.5g of isobutyramide, 0.2g of MgSO_4·7H_2O, and 0.02g of FeSO_4.7H_2O per liter of distilled water with pH controlled at 7.1, the maximum total activity was 665 units/㎖ of the culture broth and the specific activity was 70 units/㎎ of the dry cells. The medium optimization increased the specific activity of Brevibacterium sp. CH2 2.2 times.
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